Genome sequencing of E. coli O157:H7 and O55:H7 strains - SRA

SRX11717516: Genome sequencing of E. coli O157:H7 and O55:H7 strains
2 PACBIO_SMRT (PacBio RS II) runs: 326,964 spots, 3.3G bases, 11.3Gb downloads

Design: DNA was extracted with Qiagen 100/G DNA extraction kit. SMRTbell Template Prep Kit 1.0 was used to make sequencing library. Libraries were size selected to 15 kb using BluePippin. Library was sequenced using Pacbio RS II.

Submitted by: United State Department of Agriculture

Study: Escherichia coli O157:H7 genome sequencing and assembly using long-read sequencing

PRJNA528413 • SRP332089 • All experiments • All runs

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The goal of this study is to compare complete genomes from Shiga toxin-containing Escherichia coli O157:H7 strains. Many studies have used short-read sequencing technologies to obtain draft genomes for comparison. However, this study uses long-read sequencing to generate complete closed genomes for comparison. This study will provide additional information about the architecture of the chromosome along with describing the different mobile genetic elements.

Sample:

SAMN11229489 • SRS9747945 • All experiments • All runs

Organism: Escherichia coli O157:H7

Library:

Name: F6294_PacBio

Instrument: PacBio RS II

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: SINGLE

Runs: 2 runs, 326,964 spots, 3.3G bases, 11.3Gb

Run	# of Spots	# of Bases	Size	Published
SRR15415529	163,482	1.5G	5.2Gb	2021-08-16
SRR15462576	163,482	1.8G	6.1Gb	2021-08-16

ID:: 15633668

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